Sexual Health

A Nationally Representative Analysis of Substance Use and Sexual Health Correlates Associated with HIV and STI Testing among Adolescents.

TL;DR

Females engaging in substance use had increased odds of HIV and STI testing whereas that pattern did not reflect among males, highlighting the need for tailored HIV and STI testing promotion among adolescents.

Key Findings

Female adolescents engaging in substance use had increased odds of HIV and STI testing, but this pattern was not observed among male adolescents.

  • Analysis was stratified by sex to identify differential associations between substance use and testing behaviors
  • The asymmetry between male and female substance use associations with testing was a primary finding of the study
  • Data came from the 2019 and 2021 cycles of the CDC Youth Risk Behavioral Surveillance System (YRBSS)
  • Sample included n=30,909 adolescents with a mean age of 16, of whom 51% were male

Female adolescents engaging in condomless sex had lower odds of both HIV and STI testing.

  • Condom use during sex was included as a predictor variable in the multivariate logistic regression models
  • This finding suggests that females at higher behavioral risk for HIV/STIs due to condomless sex were less likely to be tested
  • Models were adjusted for race/ethnicity and age
  • The analysis used cross-sectional weighted stepwise multivariate logistic regression

Multiracial male adolescents had increased odds of HIV testing compared to other racial/ethnic groups of males.

  • Race/ethnicity was included as a covariate in all models
  • This finding was specific to male adolescents and HIV testing (not STI testing broadly)
  • The sample reflected national representation through use of YRBSS weighted data
  • The 2019 and 2021 YRBSS cycles were combined for this analysis

Predictors examined in the models included drugs and alcohol before sex, condom use, number of sex partners, sex of sexual partners, e-cigarette use, alcohol use, cannabis use frequency, lifetime cocaine use, and lifetime prescription drug misuse.

  • Cross-sectional weighted stepwise multivariate logistic regression models were used
  • Models were stratified by sex
  • All models adjusted for race/ethnicity and age
  • Both 2019 and 2021 YRBSS cycles were used, capturing pre- and during-COVID-19 pandemic data
  • Outcome variables were lifetime HIV testing and lifetime STI testing

The study sample of 30,909 adolescents had a mean age of 16 years, with 51% male, drawn from a nationally representative dataset.

  • Data sourced from the CDC Youth Risk Behavioral Surveillance System (YRBSS) 2019 and 2021 cycles
  • YRBSS is designed to be nationally representative of high school students in the US
  • Weighted analyses were conducted to maintain national representativeness
  • The study population consisted of high school students

The study concludes that HIV and STI screenings should follow the concurrent nature of sexual health and substance use behaviors of adolescents for integrated HIV/STI testing.

  • Authors recommend tailored HIV and STI testing promotion strategies
  • The study calls for ongoing efforts to dismantle barriers to testing services
  • Findings suggest integrated screening approaches that address both sexual health and substance use simultaneously
  • Sex-stratified differences in testing correlates suggest that interventions should be tailored by gender

What This Means

This research used a large, nationally representative survey of over 30,000 U.S. high school students to examine which behaviors — including substance use and sexual practices — were associated with whether teenagers had ever been tested for HIV or other sexually transmitted infections (STIs). The researchers looked separately at boys and girls, and accounted for factors like age, race, drug and alcohol use before sex, condom use, and number of sexual partners. The study found some striking differences between male and female students. For girls, using substances like alcohol, cannabis, or e-cigarettes was associated with higher likelihood of having been tested for HIV or STIs, but this same connection was not seen in boys. Perhaps more concerning, girls who reported not using condoms during sex were actually less likely to have been tested — meaning those at potentially higher risk were less likely to know their status. Among boys, those who identified as multiracial had higher odds of having been tested for HIV. This research suggests that current HIV and STI testing efforts may not be reaching all adolescents equally, and that one-size-fits-all approaches are likely insufficient. The findings point to the need for testing programs that are tailored by gender and that address substance use and sexual health together, rather than treating them as separate issues. Removing barriers to testing — particularly for males and for young people engaging in condomless sex — may be an important public health priority.

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Citation

Weerakoon S, Srikanth N, Aivadyan C, Takenaka B, Rose R, Rutledge J, et al.. (2025). A Nationally Representative Analysis of Substance Use and Sexual Health Correlates Associated with HIV and STI Testing among Adolescents.. AIDS and behavior. https://doi.org/10.1007/s10461-024-04575-w