Acute Performance, Mechanical and Thermal Effects of Isometric Conditioning Versus Standardized Volleyball Pre-Training Activation in Highly Trained Male Players.
Terbalyan A, Gepfert M, et al. • Journal of sports science & medicine • 2026
A brief maximal isometric squat protocol produced superior acute jump performance improvements compared to a standard volleyball warm-up, but only when preceded by lower training volume, with gains aligning with neuro-mechanical rather than thermal mechanisms.
Key Findings
Results
ICA produced greater CMJ height improvements than VPA specifically when delivered in Period 2 (lower preceding training volume).
The Period×Sequence interaction favored ICA for CMJ when delivered in Period 2
ΔCMJ was significantly higher under ICA versus VPA (p = 0.005, d = 1.20)
The effect size of d = 1.20 indicates a large practical difference between conditions
No significant advantage was observed when ICA was delivered in Period 1 (higher training volume)
Results
ICA produced greater relative peak power than VPA in Period 2.
Peak power was significantly greater under ICA versus VPA (p = 0.026, ηp² = 0.35)
ηp² = 0.35 represents a large effect size
This advantage was specific to the Period 2 (lower training volume) condition
The result mirrors the CMJ findings, indicating a consistent neuromuscular performance benefit
Results
ICA produced substantially more beneficial responders than VPA.
9 out of 14 participants were classified as beneficial responders under ICA versus only 1 out of 14 under VPA
McNemar exact test: p = 0.021
This represents 64% of participants responding positively to ICA compared to 7% for VPA
Responder analysis was conducted to capture individual variability in PAPE responses
Results
Reductions in rectus femoris muscle stiffness independently predicted CMJ gains following ICA in Period 2.
Standardized beta coefficient: β_std = -0.495, p = 0.005
The negative coefficient indicates that greater reductions in muscle stiffness were associated with greater CMJ improvements
This finding was derived from regression analysis at matched time points post-conditioning
Rectus femoris viscoelastic properties were assessed using muscle stiffness measurement methods
Results
Skin surface temperature changes did not predict CMJ performance gains, suggesting thermal mechanisms did not underpin the PAPE effect.
SST changes were not significantly associated with CMJ gains (p = 0.974)
SST was assessed at matched time points pre and 3, 6, 9, and 12 min post-conditioning
This null finding contrasts with the significant neuro-mechanical (stiffness) predictor
Authors concluded that gains 'align with neuro-mechanical rather than thermal mechanisms'
Results
RSImod and contraction time showed no significant differences between ICA and VPA conditions.
Neither reactive strength index modified (RSImod) nor contraction time (CT) differed significantly between conditions
CMJ metrics were assessed pre and at 3, 6, 9, and 12 minutes post-conditioning
Analyses used repeated-measures ANOVA
The null findings for RSImod and CT suggest the performance benefit was specific to jump height and peak power
Results
Preceding training volume moderated the PAPE response, with residual fatigue from higher training loads appearing to attenuate ICA benefits.
The ICA advantage emerged specifically in Period 2, which was associated with lower preceding training volume
Period×Sequence interactions were used to detect this moderating effect
The study used a randomized crossover design with 14 male volleyball players (27 ± 3 years)
Authors interpreted this as suggesting that 'residual fatigue from training may reduce the effects of PAPE'
Methods
The ICA protocol consisted of 3 sets of 5 repetitions of 3-second maximal isometric back-squat contractions, while VPA consisted of mobility and plyometric drills lasting 9 minutes.
ICA knee angle was fixed at 120°
Inter-set rest for ICA was 3 minutes
VPA included mobility and plyometric drills lasting approximately 9 minutes
Fourteen highly trained male volleyball players (27 ± 3 years) completed both conditions in a randomized crossover design
What This Means
This research suggests that a short, intense isometric squat warm-up — where athletes push maximally against an immovable bar for brief intervals — can boost jump performance more effectively than a typical volleyball warm-up routine involving mobility and plyometric drills. The study tested 14 highly trained male volleyball players in both protocols and found that the isometric approach led to meaningful improvements in countermovement jump height and power output, with 9 out of 14 players responding positively compared to only 1 out of 14 with the standard warm-up.
However, this advantage was not universal — it appeared specifically when athletes had lower training loads in the preceding session. When athletes came into the session more fatigued from prior training, the isometric protocol's benefits were diminished. This suggests that the overall fatigue state of an athlete plays an important role in determining whether a high-effort priming strategy will be effective, and coaches may need to consider training load when planning such warm-up protocols.
The study also found that improvements in jump performance were linked to reductions in muscle stiffness in the quadriceps (rectus femoris), rather than increases in muscle temperature — a common assumption about how warm-ups work. This research suggests the isometric squat protocol works through mechanical and neural pathways rather than simply by heating the muscles, and that it could serve as a practical, equipment-minimal tool for priming elite volleyball players before training or competition when fatigue levels are appropriately managed.
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Terbalyan A, Gepfert M, Szymczyk P, Koźlenia D, Lum D, Jarosz J. (2026). Acute Performance, Mechanical and Thermal Effects of Isometric Conditioning Versus Standardized Volleyball Pre-Training Activation in Highly Trained Male Players.. Journal of sports science & medicine. https://doi.org/10.52082/jssm.2026.728