Adaptogenic Effects of Mushroom Blend Supplementation on Stress, Fatigue, and Sleep: A Randomised, Double-Blind, and Placebo-Controlled Trial.

Anxiety scores showed greater reductions in the Restake group compared to placebo at both 6 and 12 weeks.

• STAI-S reductions were significant at 6 weeks (p = 0.025) and 12 weeks (p = 0.011)
• HAM-A reductions were significant at 6 weeks (p = 0.002) and 12 weeks (p = 0.002)
• 50 participants were randomly assigned to Restake or placebo groups
• Assessments were conducted at baseline, 6 weeks, and 12 weeks using validated tools

Depression scores as measured by the Beck Depression Inventory (BDI) significantly decreased in the Restake group.

• BDI scores significantly decreased at 6 weeks (p < 0.001)
• BDI scores significantly decreased at 12 weeks (p = 0.008)
• The trial was randomised, double-blind, and placebo-controlled

Fatigue levels showed significant reductions across multiple fatigue dimensions in the Restake group.

• General fatigue was significantly reduced (p = 0.043)
• Physical fatigue was significantly reduced (p = 0.027)
• Mental fatigue was significantly reduced (p = 0.043)
• Fatigue was assessed using the Visual Analog Scale for Fatigue (VAS-F) and Multidimensional Fatigue Inventory (MFI)

Sleep quality as measured by the Pittsburgh Sleep Quality Index (PSQI) improved significantly in the Restake group.

• PSQI scores were reduced at 6 weeks (p = 0.005)
• PSQI scores were reduced at 12 weeks (p < 0.001)
• Lower PSQI scores indicate better sleep quality

Serum biomarker analysis revealed significant reductions in cortisol and ACTH levels in the Restake group.

• Cortisol levels were significantly reduced (p < 0.001)
• ACTH levels were significantly reduced (p < 0.001)
• CRP levels were also significantly decreased (p = 0.042)
• Norepinephrine (NE) levels were significantly changed (p = 0.033)
• Biomarkers were analyzed via ELISA

Restake supplementation was associated with an increased morning melatonin trend after 12 weeks compared to placebo.

• The melatonin increase was described as a trend after 12 weeks of intervention
• Melatonin was measured among serum biomarkers via ELISA
• This finding was compared to the placebo group

Restake mushroom blend supplementation was well tolerated with no adverse effects reported.

• The study included 50 participants randomised to Restake or placebo
• The intervention duration was 12 weeks
• No adverse effects were reported in the Restake supplementation group