Ultrasound measurements of rectus femoris and vastus intermedius are independently associated with cardiopulmonary performance, with greater muscle thickness correlating with higher peak oxygen consumption and improved ventilatory efficiency during maximal exercise testing in male soccer athletes.
Key Findings
Results
Thigh muscle thickness measurements showed significant correlations with peak VO2 in male soccer athletes.
Rectus femoris (RF), vastus intermedius (VI), and overall muscle thickness of thigh (MT) were all significantly correlated with peak VO2
Correlations were statistically significant at p < 0.01
Measurements were obtained using muscle ultrasound (MUS)
Population consisted of competitive male soccer athletes
Results
Thigh muscle thickness measurements showed significant correlations with ventilatory efficiency (VE/VCO2 slope) in male soccer athletes.
RF, VI, and MT were all significantly correlated with VE/VCO2 slope
Correlations were statistically significant at p < 0.01
VE/VCO2 slope was assessed via cardiorespiratory exercise testing (CPET)
Greater muscle thickness was associated with improved ventilatory efficiency during maximal exercise testing
Results
Rectus femoris, vastus intermedius, and overall thigh muscle thickness were independently associated with peak VO2 in regression analysis.
Regression analysis demonstrated independent associations between RF, VI, MT and peak VO2
Associations were statistically significant at p < 0.001
Body composition parameters were also analyzed alongside ultrasound measurements
The association remained independent, suggesting muscle thickness contributes beyond general body composition measures
Results
Rectus femoris, vastus intermedius, and overall thigh muscle thickness were independently associated with VE/VCO2 slope in regression analysis.
Regression analysis demonstrated independent associations between RF, VI, MT and VE/VCO2 slope
Associations were statistically significant at p < 0.001
Both individual muscle measurements (RF and VI) and combined thigh muscle thickness (MT) showed independent associations
Physical capacity was evaluated by CPET
Background
Muscle ultrasound is described as a reliable method for quantifying regional skeletal muscle in athletes.
Previous research had shown a relationship between MUS evaluations and skeletal muscle strength
The relationship between thigh muscle thickness and cardiorespiratory capacity evaluated by CPET was previously unexplored
Rectus femoris and vastus intermedius were the specific muscles measured via ultrasound
Skeletal muscle represents a substantial portion of whole body mass in athletes
Komici K, Parente A, Di Trolio R, Femminella G, di Lorenzo C, Rengo G, et al.. (2026). Associations of thigh muscle thickness with cardiorespiratory fitness and ventilatory efficiency in male soccer athletes.. Scientific reports. https://doi.org/10.1038/s41598-026-38770-7