Dietary Supplements

Beetroot juice supplementation enhances the effects of blood flow restriction training on lower limb strength and vertical jump performance under fatigue in male university students: a randomized, double-blind, placebo-controlled study.

TL;DR

Four weeks of BFR training with or without BRJ supplementation improved knee flexor and extensor strength and bilateral CMJ performance, but BRJ effects were selective rather than broadly superior, mainly enhancing BFR training by reducing fatigue-related declines in vertical jump performance.

Key Findings

Both BFR and BFR+BRJ interventions significantly increased relative peak torque of knee extensors/flexors at 60°/s.

  • Left leg: ppre-post = 0.23, d = -0.89, ηp²Time = 0.775
  • Right leg: ppre-post = 0.017, d = -0.63, ηp²Time = 0.744
  • Training was performed at 40% limb occlusion pressure and 30% of peak torque load, three times per week for four weeks
  • Both groups (n = 10 each) showed improvements, indicating a significant time effect

Both BFR and BFR+BRJ interventions significantly increased relative peak torque of knee extensors/flexors at 180°/s.

  • Left leg: ppre-post = 0.028, d = -1.32, ηp²Time = 0.319
  • Right leg: ppre-post = 0.007, d = -1.48, ηp²Time = 0.822
  • Effect sizes were large (d > 1.0) for both legs
  • The ηp²Time for the right leg (0.822) indicated a particularly large time effect

Both BFR and BFR+BRJ interventions significantly increased maximal voluntary isometric contraction (MVIC) torque of knee extensors/flexors.

  • Left leg: ppre-post = 0.007, d = -0.11, ηp²Time = 0.825
  • Right leg: ppre-post = 0.009, d = -1.31, ηp²Time = 0.842
  • Both partial eta-squared values exceeded 0.80, indicating very large time effects
  • The right leg showed a notably larger effect size (d = -1.31) compared to the left leg (d = -0.11)

Both interventions significantly improved knee extensor torque during the 100-repetition maximal voluntary contraction fatigue test at 90°/s, for both initial and final repetitions.

  • Left leg: ppre-post = 0.029, d = -0.96, ηp²Time = 0.612
  • Right leg: ppre-post = 0.007, d = -1.21, ηp²Time = 0.725
  • Both initial (first 20 repetitions) and final (last 20 repetitions) torque values were significantly increased
  • Improvements in both early and late repetitions suggest enhanced fatigue resistance

Both BFR and BFR+BRJ interventions significantly improved fatigued bilateral countermovement jump (CMJ) height, peak force, and rate of force development (RFD).

  • Bilateral CMJ height: ppre-post = 0.048, d = -0.534, ηp²Time = 0.556
  • Peak force: ppre-post = 0.047, d = -0.913, ηp²Time = 0.444
  • Rate of force development (RFD): ppre-post = 0.044, d = -0.902, ηp²Time = 0.656
  • Single-leg CMJ performance showed no significant improvements for either the left or right leg in either group

Post-intervention, only the BFR+BRJ group showed a statistically significant improvement in fatigued bilateral CMJ height.

  • BFR+BRJ group fatigued bilateral CMJ height: ppre-post = 0.012, d = -1.307, ηp²Time = 0.846
  • This finding was described as 'notable' by the authors, distinguishing the combined intervention from BFR alone
  • The effect size was large (d = -1.307) and the partial eta-squared was very large (0.846)
  • BRJ supplementation dose was 8 mmol/day of nitrate, preceded by a one-week pre-supplementation phase

The effects of BRJ supplementation were selective rather than broadly superior to BFR training alone.

  • BRJ mainly enhanced BFR training by reducing fatigue-related declines in vertical jump performance
  • BRJ did not produce broadly superior improvements across all measured strength and performance outcomes
  • The study included 20 male university students from the School of Sports, randomly assigned to BFR (n = 10) or BFR+BRJ (n = 10) groups
  • The trial was randomized, double-blind, and placebo-controlled

The study design included a one-week BRJ pre-supplementation phase followed by a four-week bilateral knee extensor/flexor BFR training program.

  • BRJ supplementation dose: 8 mmol nitrate/day
  • BFR training parameters: 40% limb occlusion pressure, 30% of peak torque load
  • Training frequency: three sessions per week for four weeks
  • Outcomes were assessed using an isokinetic dynamometer (at 60°/s, 180°/s, and MVIC) and a force plate pre- and post-intervention
  • Participants were male university students: BFR group age 21.9 ± 1.7 years; BFR+BRJ group age 21.8 ± 1.5 years

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Citation

Yang X, Lu Y, Lee S, Xu H, Chang H, Liu Q, et al.. (2026). Beetroot juice supplementation enhances the effects of blood flow restriction training on lower limb strength and vertical jump performance under fatigue in male university students: a randomized, double-blind, placebo-controlled study.. Journal of the International Society of Sports Nutrition. https://doi.org/10.1080/15502783.2026.2636613