Lactiplantibacillus plantarum ATA-LPC98052 demonstrated favorable in vitro probiotic characteristics, technological stability, gut microbiota-modulating potential, and a favorable subacute safety profile under the tested conditions, though microbiota findings were exploratory and phenotypic MIC testing remains warranted.
ATA-LPC98052 exhibited gamma-hemolysis, indicating it does not lyse red blood cells and poses no hemolytic risk.
ATA-LPC98052 maintained substantial viability under simulated gastric acid conditions, with 60% viability at pH 1.5 and 96% viability at pH 5.0.
ATA-LPC98052 demonstrated high bile salt tolerance, with viability ranging from 74% to 82% across 0.1–0.5% bile salt concentrations.
ATA-LPC98052 showed high adhesion to Caco-2 intestinal epithelial cells exceeding 90%, with negligible cytotoxicity (99% cell viability).
The lyophilized preparation of ATA-LPC98052 was stable for 15 months, maintaining 9.6 log10 CFU/g.
Repeated oral administration of ATA-LPC98052 to Wistar rats for 28 days caused no mortality and no consistent treatment-related toxicological pattern.
Longitudinal fecal microbiota analysis showed no significant treatment × time effects on alpha diversity or community structure, and no genus-level association was FDR-significant.
Whole genome sequencing (WGS) confirmed ATA-LPC98052 identity as L. plantarum with no contamination detected, and ResFinder found no acquired antimicrobial resistance determinants meeting specified thresholds.
This research suggests that a specific probiotic bacterial strain called Lactiplantibacillus plantarum ATA-LPC98052 has favorable safety and functional characteristics that support its potential use as a probiotic ingredient. In laboratory tests, the strain survived simulated stomach acid (retaining 60–96% viability depending on pH) and bile salts (74–82% viability), adhered strongly to intestinal cells (over 90% adhesion), and did not damage those cells (99% cell viability). The freeze-dried preparation also remained stable and potent for 15 months, which is important for commercial products. When given to rats at a high dose every day for 28 days, the strain caused no deaths and no consistent signs of toxicity across blood, organ, and clinical measures. The study also used advanced genetic sequencing to confirm the strain's identity and screen for potential safety concerns. No acquired antibiotic resistance genes were found at concerning levels, though two low-similarity gene sequences (qacJ and vanY) were flagged as needing further investigation with laboratory-based antibiotic resistance testing. Analysis of the gut bacteria (microbiome) in the rats showed that taking this probiotic did not significantly disrupt the overall balance or diversity of the gut microbial community, which is generally considered a positive safety indicator. This research suggests that ATA-LPC98052 has the characteristics expected of a safe and functional probiotic candidate, but the authors emphasize that some findings — particularly around antibiotic resistance and gut microbiota effects — are preliminary and require additional testing before definitive conclusions can be drawn. Because probiotic safety is strain-specific, these results apply only to this particular strain and cannot be generalized to other L. plantarum products.
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Upload Your LabsKurtuluş &, Bursalıoğlu E, Aliusta R, Türker Şallı B, Özkan B, Köşebent D, et al.. (2026). Comprehensive In Vitro, Genomic, Microbiota, and Subacute Toxicological Safety Characterization of Lactiplantibacillus plantarum ATA-LPC98052.. Nutrients. https://doi.org/10.3390/nu18172796