Aging & Longevity

Dapagliflozin attenuates cellular senescence and mitochondrial dysfunction in diabetic kidney disease: potential involvement of AMPK/Sirt3 signaling.

TL;DR

Dapagliflozin attenuates renal cellular senescence and improves mitochondrial function in diabetic kidney disease through involvement of AMPK/Sirt3 signaling, as evidenced by attenuation of dapagliflozin-mediated protection following AMPK inhibition and knockdown of Sirt3.

Key Findings

Dapagliflozin reduced the expression of renal senescence markers in both in vivo (streptozotocin-induced diabetic mice) and in vitro (high glucose-treated HK-2 cells) experiments.

  • Streptozotocin-induced diabetic mice were used as the in vivo model of diabetic kidney disease (DKD).
  • High glucose-treated human renal tubular epithelial (HK-2) cells served as the in vitro model.
  • Senescence markers were measured across both experimental systems to confirm the anti-senescence effect of dapagliflozin.
  • The renoprotective effects were observed independent of glucose-lowering effects.

Dapagliflozin improved mitochondrial function across multiple parameters in DKD models.

  • Evidence of mitochondrial improvement included restoration of mitochondrial membrane potential.
  • Dapagliflozin also improved mitochondrial dynamics.
  • Mitochondrial reactive oxygen species (ROS) levels were reduced following dapagliflozin treatment.
  • Levels of senescence-associated secretory phenotypes (SASPs) were also lowered by dapagliflozin.

AMPK inhibition with Compound C attenuated AMPK phosphorylation and Sirt3 expression, consequently reducing dapagliflozin-mediated protective effects.

  • Compound C was used as a pharmacological AMPK inhibitor in HK-2 cell experiments.
  • AMPK phosphorylation was reduced upon Compound C treatment, indicating AMPK pathway involvement.
  • Sirt3 expression level was also attenuated by Compound C, suggesting Sirt3 acts downstream of AMPK.
  • The protective effects of dapagliflozin against cellular senescence and mitochondrial dysfunction were consequently attenuated when AMPK was inhibited.

Knockdown of Sirt3 in HK-2 cells substantially reduced dapagliflozin's improvements on mitochondrial function and its protective effects against cellular senescence.

  • Sirt3 knockdown was achieved by small interfering RNA (siRNA) transfection in HK-2 cells.
  • Sirt3 knockdown reversed dapagliflozin's improvements on mitochondrial function.
  • Sirt3 knockdown also reversed dapagliflozin's protective effects against cellular senescence.
  • Sirt3 knockdown reversed dapagliflozin's alleviation of kidney function, implicating Sirt3 as a necessary mediator of dapagliflozin's renoprotective effects.

Mitochondrial dysfunction and cellular senescence were identified as closely associated pathological processes influencing one another during kidney injury progression in DKD.

  • The study was designed to explore whether dapagliflozin's renal protective mechanism operates via modulating cellular senescence, an area previously poorly understood.
  • Cellular senescence was identified as contributing to the pathology of diabetic kidney disease.
  • The interaction between mitochondrial dysfunction and cellular senescence was examined as a mechanistic focus of the study.

What This Means

This research suggests that dapagliflozin, a diabetes medication commonly used to lower blood sugar, also protects the kidneys through a mechanism unrelated to glucose control. Specifically, the study found that dapagliflozin reduces 'cellular senescence' — a process where cells stop functioning properly and enter a harmful aging-like state — in kidney cells of diabetic mice and in human kidney cells grown in high-sugar conditions in the laboratory. At the same time, the drug appeared to restore the health of mitochondria, the energy-producing structures inside cells, by improving their electrical charge, reducing harmful reactive oxygen species, and improving how mitochondria divide and fuse. The research also identified a molecular pathway — involving proteins called AMPK and Sirt3 — that appears to be responsible for these protective effects. When scientists blocked AMPK with a chemical inhibitor or silenced the Sirt3 gene using a genetic technique, the kidney-protective benefits of dapagliflozin were substantially reduced. This suggests that dapagliflozin works, at least in part, by activating AMPK, which in turn increases Sirt3 activity, leading to better mitochondrial health and less cellular senescence in the kidney. These findings matter because diabetic kidney disease is a leading cause of kidney failure worldwide, and current treatments have limited ability to stop its progression. This research suggests that dapagliflozin's ability to slow kidney disease may go beyond controlling blood sugar and may involve protecting kidney cells from premature aging and energy failure, opening potential new directions for understanding and treating this condition.

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Citation

Tong X, Wang B, Gao F, Zhong A, Fan W, Zhang Y, et al.. (2026). Dapagliflozin attenuates cellular senescence and mitochondrial dysfunction in diabetic kidney disease: potential involvement of AMPK/Sirt3 signaling.. Biochemical pharmacology. https://doi.org/10.1016/j.bcp.2026.118382