Cardiovascular

Defective efferocytosis links autonomic dysfunction and atrial fibrillation: multi-omics integration and in vivo validation.

TL;DR

Multi-omics integration and in vivo validation suggest an ANS-immune-atrial remodeling axis in which CD47-SIRPα-dependent efferocytosis blockade is highly associated with atrial inflammation and AF susceptibility, with valsartan acting as a clinically relevant modulator of this pathway.

Key Findings

Plasma from AF patients exhibited an autonomic-imbalance signature characterized by upregulated monoaminergic and downregulated cholinergic neurotransmitters.

  • Targeted LC-MS/MS profiling of 39 neurotransmitters was conducted in a clinical cohort of AF patients
  • Monoaminergic neurotransmitters (sympathetic branch) were found to be upregulated in AF patient plasma
  • Cholinergic neurotransmitters (vagal branch) were found to be downregulated in AF patient plasma
  • This pattern is consistent with an imbalance between sympathetic and vagal branches of the autonomic nervous system (ANS)

Bioinformatic integration of atrial transcriptomes and a sympathetic neuron activation dataset identified four hub genes shared between ANS dysregulation and AF: CDKN2D, FYTTD1, LRR1, and POPDC3.

  • Analysis integrated atrial transcriptomes with a sympathetic neuron activation dataset
  • Methods included differentially expressed genes (DEGs), WGCNA, enrichment analysis, immune infiltration, and machine-learning feature selection
  • Four hub genes were identified: CDKN2D, FYTTD1, LRR1, and POPDC3
  • In silico docking was also performed as part of the bioinformatic pipeline

Immune analyses identified CD47-mediated efferocytosis as a crucial mechanistic link between autonomic nervous system dysfunction and atrial fibrillation.

  • Immune infiltration analyses were used to pinpoint the CD47-SIRPα signaling axis
  • CD47 is a 'don't eat me' signal that inhibits macrophage-mediated clearance of apoptotic cells (efferocytosis)
  • This finding emerged from multi-omics integration of the shared ANS-AF bioinformatic analysis
  • CD47-SIRPα-dependent efferocytosis blockade was identified as highly associated with atrial inflammation and AF susceptibility

Angiotensin II (Ang II) infusion in mice induced autonomic remodeling, calcium-handling suppression, increased apoptosis with CD47/SIRPα upregulation, impaired efferocytosis, and heightened inflammatory signaling.

  • An Ang II-infused mouse model was used for in vivo validation
  • Outcome measures included transesophageal burst pacing, immunostaining, immunoblotting, and efferocytosis quantification
  • Ang II induced upregulation of both CD47 and SIRPα
  • Ang II also caused Ca²⁺-handling suppression and increased apoptosis alongside impaired efferocytosis
  • These changes collectively represent an ANS-immune-atrial remodeling axis

Valsartan intervention partially reversed Ang II-induced autonomic remodeling, CD47/SIRPα upregulation, impaired efferocytosis, and inflammatory signaling in the mouse model.

  • Valsartan (an angiotensin receptor blocker) was used as an intervention in the Ang II-infused mouse model
  • Valsartan partially reversed changes in autonomic remodeling induced by Ang II
  • Valsartan partially reversed Ca²⁺-handling suppression, increased apoptosis, CD47/SIRPα upregulation, impaired efferocytosis, and heightened inflammatory signaling
  • The paper describes valsartan as 'a clinically relevant modulator of this pathway'
  • Reversal was described as partial, not complete

What This Means

This research suggests that atrial fibrillation (AF), the most common serious heart rhythm disorder, may be connected to immune system dysfunction through an unexpected pathway involving the clearance of dying cells. The study found that AF patients show a chemical imbalance in their nervous system — with too much activity from the 'fight-or-flight' (sympathetic) branch and too little from the 'rest-and-digest' (vagal) branch. By combining patient blood data, heart tissue gene expression, and computer-based analyses, the researchers identified that a molecule called CD47 — which acts as a 'don't eat me' signal on dying cells — may block immune cells (macrophages) from cleaning up dead cells in the heart, a process called efferocytosis. This blockade appears to drive inflammation in the heart's upper chambers, potentially promoting AF. To test this idea in a living system, the researchers treated mice with a hormone called angiotensin II, which mimics some features of cardiovascular stress. These mice developed nerve remodeling in the heart, problems with calcium handling (important for normal heart rhythm), more cell death, and a buildup of the CD47 signal that prevented proper cellular cleanup. When mice were treated with valsartan — a blood pressure medication already used in clinical practice — many of these harmful changes were partially reversed, including the impaired efferocytosis and inflammation. This research suggests a chain of events linking nervous system imbalance → impaired clearance of dying heart cells → inflammation → AF susceptibility. The findings are significant because they point to efferocytosis (cellular cleanup) as a potential new target for AF treatment, and they suggest that existing drugs like valsartan may already be working partly through this mechanism. However, because this study used mouse models and bioinformatics in addition to human samples, further clinical research would be needed to confirm these findings in patients.

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Citation

Zhang X, Du Y, Shi S, Liu Y, Ye Z, Chai R, et al.. (2026). Defective efferocytosis links autonomic dysfunction and atrial fibrillation: multi-omics integration and in vivo validation.. Frontiers in immunology. https://doi.org/10.3389/fimmu.2026.1818859