Distinct Gut Microbiome Profiles Underlying Cardiometabolic Risk Phenotypes in Individuals with Obesity.

Reduced relative abundance of Lachnospiraceae was associated with metabolic syndrome in individuals with obesity.

• Study used a cross-sectional design with 100 adults with obesity stratified according to metabolic syndrome status.
• Gut microbiome composition was assessed using targeted multiplex real-time PCR of functionally relevant bacterial taxa.
• ROC analyses identified cohort-specific discriminative thresholds with moderate accuracy for Lachnospiraceae.
• Associations were examined using correlation analysis, ROC curves, and multivariable logistic regression models.

Lower Faecalibacterium abundance was associated with arterial hypertension in individuals with obesity.

• Finding was identified in a cohort of 100 adults with obesity using targeted multiplex real-time PCR.
• ROC analyses identified cohort-specific discriminative thresholds with moderate accuracy for Faecalibacterium.
• Multivariable logistic regression models were used to examine the association.
• The association was identified as part of broader investigation of gut bacterial taxa and cardiometabolic risk phenotypes.

Increased Prevotella abundance was associated with dyslipidemia in individuals with obesity.

• Finding was identified in a cohort of 100 adults with obesity using targeted multiplex real-time PCR.
• ROC analyses identified cohort-specific discriminative thresholds with moderate accuracy for Prevotella.
• Associations with anthropometric and cardiometabolic parameters were examined using correlation analysis, ROC curves, and multivariable logistic regression models.
• The authors describe these findings as exploratory and requiring validation in longitudinal and independent cohorts.

ROC analyses identified cohort-specific discriminative thresholds for gut bacterial taxa with moderate accuracy.

• ROC curves were applied to evaluate discriminative capacity of Lachnospiraceae, Faecalibacterium, and Prevotella for their respective cardiometabolic risk phenotypes.
• The accuracy of these thresholds was characterized as moderate.
• Analyses were conducted in a cross-sectional sample of 100 adults with obesity.
• The authors note findings are exploratory and require validation in longitudinal and independent cohorts.

Obesity-related cardiometabolic disorders have been linked to alterations in selected gut microbiome components, yet clinically relevant microbial signatures remain incompletely defined.

• The study was motivated by incomplete definition of clinically relevant microbial signatures in obesity.
• The study investigated associations between selected gut bacterial taxa and cardiometabolic risk phenotypes.
• A targeted multiplex real-time PCR approach was used to assess functionally relevant bacterial taxa rather than broad metagenomic sequencing.
• Participants were stratified according to metabolic syndrome status to define cardiometabolic risk phenotypes.