Multimodal functional training over 10 weeks led to an improved ILA/IAA index suggesting a neuroprotective shift in gut microbiota composition, and a single bout acutely increases the circulating level of ILA in persons with relapsing-remitting multiple sclerosis.
Key Findings
Results
Baseline indole levels in RRMS patients differed from those of matched healthy controls, with reduced ILA levels observed in RRMS patients.
Blood samples from RRMS patients at baseline were compared to a matched healthy control group.
Indole-3-lactate (ILA) was specifically identified as reduced in persons with MS at baseline.
Serum indole concentrations were measured using LC-MS/MS methodology.
This finding aligns with prior literature suggesting ILA is reduced in persons with MS.
Results
A 10-week multimodal functional training intervention increased the ILA/IAA (neuroprotection) index in RRMS patients.
Thirty-one RRMS patients with at least 70% session attendance completed the intervention.
The intervention consisted of 60-minute sessions, 3 times per week, over 10 weeks.
The ILA/IAA index is considered a neuroprotection index reflecting gut microbiota composition.
The training group was compared to a waitlist control group in a randomized controlled trial design (DRKS00017091).
The increase in ILA/IAA index suggests a neuroprotective shift in gut microbiota composition.
Results
A single bout of exercise acutely increased both circulating ILA levels and the ILA/IAA index in RRMS patients.
Acute effects were assessed with blood samples collected before, during, and immediately after one interim training session.
Both ILA concentration and the ILA/IAA index were elevated following the single exercise bout.
This acute response was observed within the context of the multimodal functional training program.
Serum indole concentrations were measured using LC-MS/MS.
Background
Indole-3-lactate has anti-inflammatory and remyelinating properties that may influence the gut-microbiota-brain axis in multiple sclerosis.
ILA is a tryptophan-derived metabolite produced by the gut microbiota.
ILA improves MS clinical scores in animal models via its anti-inflammatory remyelinating properties.
The ILA/IAA (indole-3-acetate) index is considered a neuroprotection index.
Physical exercise and diet can modify gut microbiota and indole metabolism.
Methods
The study design was a secondary analysis of a randomized controlled trial comparing multimodal functional training to a waitlist control group in RRMS patients.
Trial registration number: DRKS00017091.
Thirty-one RRMS patients met the inclusion criterion of at least 70% session attendance.
The intervention was 10 weeks of multimodal functional training (60 min, 3×/week).
Blood samples were collected at baseline, after 10 weeks, and acutely around one interim training session.
Baseline samples were also compared to a matched healthy control group.