Green tea's impact on fertility hormones and oxidative stress markers in obese males with different gonadal statuses in Onitsha, Nigeria.

Obese males had significantly higher oxidative stress and lower antioxidant capacity compared to normal-weight controls at baseline.

• Obese participants had significantly higher MDA (malondialdehyde) than controls (p<0.05)
• Obese participants had significantly lower TAC (total antioxidant capacity) than controls (p<0.05)
• Obese participants had significantly lower GPx (glutathione peroxidase) than controls (p<0.05)
• Controls were normal-weight eugonadic males; obese participants were aged 29-50 with n=100 total

Green tea supplementation significantly reduced the oxidative stress marker malondialdehyde in obese males.

• MDA levels were significantly reduced following 12 weeks of green tea supplementation (p<0.05)
• Participants consumed two bags of green tea infused in 150 mL of boiled water daily for 12 weeks
• Blood samples were collected at baseline, 1 month, and 2 months to track changes
• MDA was analysed colourimetrically

Green tea supplementation significantly increased total antioxidant capacity and glutathione peroxidase activity in obese males.

• TAC was significantly increased post-supplementation (p<0.05)
• GPx was significantly increased post-supplementation (p<0.05)
• Both markers were measured colourimetrically
• The intervention duration was 12 weeks with measurements at three time points

Green tea supplementation significantly reduced oestradiol and prolactin levels in obese males.

• Oestradiol was significantly reduced following green tea supplementation (p<0.05)
• Prolactin was significantly reduced following green tea supplementation (p<0.05)
• Hormonal levels were measured using ELISA
• Elevated oestradiol and prolactin are associated with obesity-related reproductive dysfunction

Green tea supplementation significantly increased testosterone levels in obese males.

• Testosterone levels were significantly increased post-supplementation (p<0.05)
• Testosterone was measured using ELISA
• The increase in testosterone occurred across obese male groups with varying gonadal statuses
• Participants were categorised into Eugonadism, Compensatory Hypogonadism, and Hypergonadotropic Hypogonadism groups

LH and FSH were normalised by green tea supplementation in Eugonadic and Compensatory Hypogonadic groups but remained elevated in Hypergonadotropic Hypogonadism.

• LH and FSH levels were normalised in the Eugonadic group following supplementation
• LH and FSH levels were normalised in the Compensatory Hypogonadic group following supplementation
• LH and FSH remained elevated in the Hypergonadotropic Hypogonadism group, indicating a differential response based on gonadal status
• LH and FSH were measured using ELISA at baseline, 1 month, and 2 months