Hypolipidemic activity of Limosilactobacillus fermentum SHY0004 from Miao sour soup.

A lactic acid bacterium was successfully isolated from Miao sour soup and identified as Limosilactobacillus fermentum SHY0004.

• The strain was isolated and purified from Miao sour soup, a traditional fermented food from Guizhou Province, China.
• The bacterium was identified as consistent with the List of Edible Microorganisms.
• The species is Limosilactobacillus fermentum, designated strain SHY0004.

L. fermentum SHY0004 exhibited feruloyl esterase activity.

• Feruloyl esterase activity was detected as part of functional evaluation of the strain.
• This enzymatic activity is noted as a relevant functional characteristic of the isolate.
• Feruloyl esterase activity is described alongside hypolipidemic effects as part of the strain's functional profile.

SHY0004 demonstrated significant hypolipidemic effects in a high-fat-induced HepG2 cell model.

• HepG2 cells were used as an in vitro liver cell model induced with high-fat conditions.
• The strain showed significant lipid-lowering activity in this cell-based assay.
• This represents one of multiple experimental models in which hypolipidemic activity was evaluated.

SHY0004 demonstrated significant hypolipidemic effects in a high-fat zebrafish model.

• A high-fat zebrafish model was employed as an in vivo experimental system.
• The strain showed significant hypolipidemic effects in this model.
• This zebrafish model served as an intermediate in vivo system between cell culture and mammalian models.

SHY0004 effectively regulated total cholesterol (TC) and triglyceride (TG) levels in serum and liver of high-fat diet mice.

• A high-fat diet (HFD) mouse experiment was conducted to evaluate the strain's hypolipidemic activity in a mammalian model.
• SHY0004 administration resulted in regulation of both TC and TG levels.
• Lipid regulation was observed in both serum and liver compartments.
• The treatment resulted in 'a clear lipid-lowering effect' in HFD mice.

Miao sour soup contains diverse beneficial microorganisms with potential physiological activities relevant to metabolic health.

• Miao sour soup is a traditional fermented food from Guizhou Province, China.
• Prior to this study, the hypolipidemic function of Miao sour soup had 'not yet been fully explored.'
• The isolation of SHY0004 provides 'new microbial resources for the development of functional probiotics.'