Lymphocyte subpopulations in healthy Ukrainian men: local reference ranges, long temporal stability, and exercise-induced variability.

Reference ranges for lymphocyte subpopulations were established for healthy Ukrainian adult men using flow cytometry.

• 100 clinically healthy men aged 22-55 years were analyzed
• CD3+ range: 54.0-82.2%
• CD3+CD4+ range: 29.3-54.0%
• CD3+CD8+ range: 11.2-39.6%
• CD3-CD19+ range: 3.7-20.1%
• CD3-CD56+ range: 4.2-30.0%; CD3-CD56+NKp46+ range: 2.7-24.3%

The established Ukrainian reference ranges differed from published studies and from reference values used by local laboratories across multiple measures.

• Comparison with published studies revealed differences in several parameters
• Discrepancies relative to reference values used by local laboratories were observed across multiple measures
• The authors conclude these differences underscore the need for harmonized, population-based reference intervals

Lymphocyte subset distributions remained largely stable over a one-year Antarctic expedition despite a significant expansion of monocytes.

• 24 participants were monitored during a one-year Antarctic expedition
• Temporal stability was evaluated over the full duration of the expedition
• Lymphocyte subset distributions were largely stable
• A significant expansion of monocytes was observed despite lymphocyte stability

Short-term physical exercise elicited pronounced, subset-selective increases in NK cells and monocytes.

• 19 men were assessed before and after a modified Cooper test
• NK cells showed pronounced increases following exercise
• Monocytes also showed pronounced increases following exercise
• Concurrent elevations in cortisol and testosterone levels were observed following exercise
• The response was described as subset-selective, with NK cells and monocytes being the primary responding populations