Cardiovascular

Pulsed Field Versus Cryoballoon Ablation for Atrial Fibrillation: Procedural Outcomes and Hemolysis-Related Laboratory Changes in a Single-Center Pilot Study.

TL;DR

Both pulsed field ablation and cryoballoon ablation caused acute declines in hemoglobin, and total bilirubin rose more after PFA, with no accompanying change in renal function.

Key Findings

Both PFA and CRYO caused substantial reductions in hemoglobin following the procedure with no statistically significant between-group difference.

  • Day-1 hemoglobin change was -1.84 g/dL in the PFA group versus -1.57 g/dL in the CRYO group.
  • The between-group difference was -0.27 g/dL (95% CI -1.01 to +0.47).
  • Group × time interaction p = 0.192, indicating no significant differential effect between modalities.
  • Study included 17 PFA patients and 16 CRYO patients in a retrospective single-center design.

Total bilirubin increased to a significantly greater extent following PFA compared to CRYO.

  • Day-1 total bilirubin change was +0.48 mg/dL in the PFA group versus +0.15 mg/dL in the CRYO group.
  • The between-group difference was +0.33 mg/dL (95% CI +0.06 to +0.59).
  • Group × time interaction p = 0.003, indicating a statistically significant differential effect.
  • The largest separation between groups occurred immediately after ablation: 1.43 ± 0.36 mg/dL (PFA) versus 0.99 ± 0.35 mg/dL (CRYO), p = 0.001.

No patient in either group met KDIGO criteria for acute kidney injury (AKI) of any stage following ablation.

  • AKI was defined using KDIGO serum creatinine criteria and assessed individually.
  • The largest post-procedural rise in creatinine was +0.10 mg/dL in both groups.
  • Sensitivity analyses were restricted to pulmonary-vein-isolation (PVI)-only procedures.
  • Renal function showed no clinically meaningful change in either the PFA or CRYO group.

Baseline demographics and clinical characteristics did not differ significantly between the PFA and CRYO groups.

  • The study included 33 symptomatic AF patients total: 17 in the PFA group and 16 in the CRYO group.
  • The study was a retrospective single-center pilot analysis.
  • Laboratory parameters were measured at three pre-specified time points: baseline, immediately after ablation, and on day 1.
  • Group × time interactions were tested using linear mixed-effects models with changes reported with 95% confidence intervals.

The authors concluded that larger studies with direct hemolysis biomarkers are needed to corroborate the finding of greater bilirubin elevation following PFA.

  • The current study was described as a pilot study with a small sample size of 33 patients.
  • Direct hemolysis biomarkers (such as lactate dehydrogenase or free hemoglobin) were not measured in this study.
  • The bilirubin elevation pattern in the PFA group was identified as compatible with hemolysis but not confirmed by dedicated hemolysis assays.
  • The authors noted emerging concerns regarding hemolysis following PFA as the motivation for the study.

What This Means

This research examined two common procedures used to treat atrial fibrillation (an irregular heart rhythm): pulsed field ablation (PFA), which uses electrical pulses to destroy abnormal heart tissue, and cryoballoon ablation (CRYO), which uses freezing. The study followed 33 patients at a single hospital, measuring blood markers before the procedure, immediately after, and the following day. Both procedures caused a notable drop in hemoglobin (a protein in red blood cells), but the drop was similar between the two groups. However, bilirubin — a substance released when red blood cells break down — rose significantly more in patients who had PFA, suggesting that PFA may cause more red blood cell destruction (hemolysis) than CRYO. Despite the bilirubin difference, neither procedure caused kidney damage as defined by established clinical criteria (KDIGO), and creatinine levels (a marker of kidney function) barely changed in either group. This is notable because one concern with hemolysis is that the byproducts of red blood cell breakdown could potentially harm the kidneys, but this was not observed in this small study. This research suggests that PFA may produce a greater degree of red blood cell breakdown than cryoballoon ablation, as reflected in higher bilirubin levels, though no kidney injury was detected in either group. Because the study was small (33 patients) and did not directly measure hemolysis with specialized tests, the authors emphasize that larger studies using dedicated hemolysis biomarkers are needed to confirm these findings and better understand the clinical significance of this difference between ablation techniques.

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Citation

Kim K, Hwang Y, Son Y, Kim J, Roh S. (2026). Pulsed Field Versus Cryoballoon Ablation for Atrial Fibrillation: Procedural Outcomes and Hemolysis-Related Laboratory Changes in a Single-Center Pilot Study.. Medicina (Kaunas, Lithuania). https://doi.org/10.3390/medicina62081580