Seminal Redox Improvement and Sperm Proteome Remodeling After Deprox-HP Nutraceutical Supplementation in Male Accessory Gland Inflammation: A Pilot Study.

Total and progressive sperm motility increased significantly after 3 months of Deprox-HP treatment in MAGI patients.

• Study included twenty MAGI patients treated for 3 months with a Graminex-based dietary supplement (Deprox-HP)
• Total sperm motility and progressive sperm motility both showed statistically significant increases after treatment
• Sperm concentration and sperm morphology showed a non-significant upward trend
• This was a pilot study with pre- and post-treatment assessment of the same patients

Sperm lipid peroxidation decreased markedly after Deprox-HP treatment, while antioxidant capacity showed a non-significant increase.

• Lipid peroxidation was assessed as a measure of oxidative membrane damage
• The decrease in sperm lipid peroxidation was described as 'marked'
• Antioxidant capacity increased after treatment but did not reach statistical significance
• Oxidative stress assessment was integrated with conventional semen analysis and sperm proteomics

Sperm proteome analysis demonstrated a clear PRE-POST clustering consistent with treatment-associated remodeling.

• Proteomic analysis was performed on sperm samples before and after 3-month Deprox-HP treatment
• PRE and POST samples clustered separately, indicating a distinct treatment-associated proteomic shift
• POST samples showed upregulation of proteins linked to sperm motility, redox homeostasis, mitochondrial metabolism, and membrane remodeling
• Proteomic methodology was integrated with conventional semen analysis and oxidative stress assessment

Two pregnancies occurred during the treatment period, with both cases showing decreased lipid peroxidation, increased morphologically typical spermatozoa, and post-treatment proteomic shifts enriched in flagellar and mitochondrial respiratory/redox compartments.

• Two pregnancies occurred among the twenty MAGI patients during the 3-month treatment period
• Both pregnancy cases showed a decrease in lipid peroxidation and an increase in morphologically typical spermatozoa post-treatment
• Sperm proteomics in both cases showed a concordant post-treatment shift enriched in flagellar and mitochondrial respiratory/redox compartments
• Both cases showed selective enrichment of TEX50, described as 'a crucial protein involved in acrosome/head-stability during epididymal transit'

TEX50, a protein involved in acrosome/head-stability during epididymal transit, was selectively enriched post-treatment in the two patients who achieved pregnancy.

• TEX50 enrichment was observed specifically in the post-treatment samples of the two patients in whom pregnancy occurred
• TEX50 is described as 'a crucial protein involved in acrosome/head-stability during epididymal transit'
• This selective enrichment was noted as distinct from the broader proteomic shifts seen across all twenty patients
• The finding was reported in the context of flagellar and mitochondrial respiratory/redox compartment enrichment in these two cases

Male accessory gland inflammation (MAGI) can impair male fertility through inflammation-driven oxidative stress and direct sperm damage, and nutraceutical approaches may be useful when antibiotics are not indicated.

• MAGI was identified as the condition under study, affecting seminal redox status and sperm quality
• The study rationale included scenarios where antibiotic treatment is not indicated
• Deprox-HP is described as a Graminex-based dietary supplement
• The study was designed as a pilot study with n=20 patients