Two'-Fucosyllactose, a Human Milk Oligosaccharide, Promotes Intestinal Remodeling and Enhances Nutrient and Functional Component Absorption.

Oral 2'FL supplementation for 20 days significantly increased the length of the small intestine in mice.

• Mice were orally supplemented with 2'FL daily for 20 days
• Small intestinal length was significantly increased compared to control groups
• No adverse effects on intestinal barrier function were observed alongside structural remodeling

2'FL supplementation significantly increased the density of villi in the small intestine.

• Villus density was measured as part of intestinal remodeling assessment
• The increase in villus density was observed after 20 days of daily oral 2'FL administration
• The structural changes were accompanied by no adverse effects on intestinal barrier function

2'FL-treated mice exhibited enhanced absorption of triglycerides containing docosahexaenoic acid (DHA) in lipid tolerance tests.

• DHA-containing triglyceride absorption was assessed via lipid tolerance tests
• Enhanced absorption was observed in mice receiving 20 days of 2'FL supplementation
• This finding indicates functional improvement in lipid absorption capacity associated with intestinal remodeling

Co-administration of 2'FL with CL316,243 significantly increased absorption of CL316,243.

• CL316,243 is a beta-3 adrenergic receptor agonist used as a functional compound in this study
• Absorption was significantly increased in the co-administration group compared to controls
• Elevated uncoupling protein 1 (UCP1) levels in inguinal adipose tissue were observed alongside increased CL316,243 absorption

Co-administration of 2'FL with curcumin significantly increased curcumin absorption.

• Curcumin absorption was significantly increased in the 2'FL co-administration group
• The enhancement in curcumin absorption was associated with the intestinal remodeling promoted by 2'FL
• These results were observed following 20 days of daily oral 2'FL supplementation

Increased CL316,243 absorption associated with 2'FL supplementation was accompanied by elevated uncoupling protein 1 (UCP1) levels in inguinal adipose tissue.

• UCP1 levels were measured in inguinal adipose tissue as a downstream functional marker
• Elevated UCP1 was observed in the 2'FL plus CL316,243 co-administration group
• This finding suggests that enhanced intestinal absorption from 2'FL remodeling translates to downstream physiological effects

2'FL supplementation did not adversely affect intestinal barrier function despite promoting structural remodeling.

• Intestinal barrier function was assessed alongside morphological measurements
• No adverse effects were detected following 20 days of daily oral 2'FL administration
• This indicates that the pro-remodeling effects of 2'FL are compatible with maintained barrier integrity