Whole-component soymilk prepared based on high-energy fluidic microfluidization alleviates ulcerative colitis by modulating gut microbiota and regulating metabolic pathways.

Whole-component soymilk (WSM) prepared by high-energy fluidic microfluidization exhibited elevated in vitro antioxidant activity compared to traditionally processed soymilk (TSM).

• Antioxidant activity in vitro was elevated by 15% in WSM compared to TSM.
• HEFM processing retained all nutrients in soybeans by producing unfiltered whole-component soymilk.
• TSM served as the comparison benchmark for antioxidant activity measurements.

WSM intervention both before and after the onset of colitis alleviated clinical symptoms of ulcerative colitis.

• WSM was evaluated at two intervention stages: before and following the onset of colitis.
• Clinical symptoms were alleviated in both intervention timing groups.
• Inflammatory factors in WSM-treated groups were closer to the normal group compared to TSM-treated groups.

WSM alleviated colon damage by increasing the expression of tight junction proteins.

• Tight junction protein expression was increased following WSM intervention.
• This effect contributed to protection of the intestinal epithelial barrier.
• The effect was observed both in pre-colitis and post-colitis intervention groups.

WSM intervention enhanced the intestinal microbial barrier by modulating gut microbiota composition.

• WSM promoted the colonization of Bacillota.
• WSM reduced the proliferation of Bacteroidota and Pseudomonadota.
• These microbial modulations were observed at both intervention stages (before and after colitis onset).
• Both WSM and TSM were compared for their effects on gut microbiota.

WSM intervention at different stages regulated colonic metabolic pathways in colitis mice.

• Colonic metabolic pathways were regulated by WSM intervention both before and following colitis.
• Metabolic pathway regulation was assessed in mouse models of colitis.
• WSM intervention was evaluated at two distinct timepoints relative to colitis onset.